>Human Nkx-2.5 
MFPSPALTPTPFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEAYAGPEAAAPGLPELR
AELGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKKGCELPRGQRPPVLFSSALSQPDFLQMLSE
TCRWLPVHLAE

>Gorilla Nkx-2.5
MFPSPALTPTPFSVKDILNLEQQQRSLAAAGELSARLEATLAPSSCMLAAFKPEAYAGPEAAAPGLPELR
ADLGRAPSPAKCASAFPAAPAFYPRAYSDPDPAKDPRAEKKGCELPRGQRPPVLFSSALSQPDFLQMLSE
TCRWLPVYLAE

>Mouse Nkx-2.5 
MFPSPALTPTPFSVKDILNLEQQQRSLASGDLSARLEATLAPASCMLAAFKPEAYSGPEAAASGLAELRA
EMGPAPSPPKCSPAFPAAPTFYPGAYGDPDPAKDPRADKKELCALQKAVELDKAETDGAERPRARRRRKP
RVLFSQAQVYELERRFKQQRYLSAPERDQLASVLKLTSTQVKIWFQNRRYKCKRQRQDQTLELLGPPPPP
ARRIAVPVLVRDGKPCLGDPAAYAPAYGVGLNAYGYNAYPYPSYGGAACSPGYSCAAYPAAPPAAQPPAA
SANSNFVNFGVGDLNTVQSPGMPQGNSGVSTLHGIRAW

>Drosophila tinman
MLQHHQQQAQSGGYYDHYTQSPSPGSLTNADALNTTPFSVKDILNMVNQTEAYEGSYGHIDGAATASALF
AAGEYQNPHQYLNHQQHQQSELPIPQQQLHHQHLDDGATTSSSLSPLLPPPPHQLYGGYQDYGMPAHMFQ
HHHGHPHQSFQHSASAYNMSASQFYAGASATAYQTPATYNYNYAGSGEVYGGATPSAVGIKSEYIPTPYV
TPSPTLDLNSSAEVDSLQAPTQKLCVNPLSQRLMETASNSSSLRSIYGSDEGAKKKDNSQVTSSRSELRK
NSISGNSNPGSNSGSTKPRMKRKPRVLFSQAQVLELECRFRLKKYLTGAEREIIAQKLNLSATQVKIWFQ
NRRYKSKRGDIDCEGIAKHLKLKSEPLDSPTSLPPPIPNHVMWPPTMQQSQQQQQHHAQQQQMQHM

>Zebrafish Nkx-2.5 
MAMFSSQMTSTPFSVRDILNLEQNQEDMVSLDMSQRLDSALIPTSSCMLSTFKQEQFMEMPSGSSLFSED
LQEDKGNKINSLNFSASGFYAKNFLEMDYVKDAKTDDTFEDKEKKDIGFCQEDPGEDLKLDDAERPKQRK
RRKPRVLFSQAQVYELERRFKQQKYLSAPERDHLANVLKLTSTQVKIWFQNRRYKCKRQRQDQTLEMVGI
APPRRISVPVLVRDGKPCLGDTSTYNTSYNVGINHFTYNTYPAFSNFPSPGNSNYSCNYPSSMSSIQPSQ
SNSNYMNFGVGDLNNVQASFQSSSVPSLHGIRAW

>Roundworm Nkx-2.5
MFNVSALAAATPSIASVSSVASPSEQHGLSTSVGVGVNDTTSRTGDGGAASSASSASAAPQQQSQSALHN
KLEAKWDTLLPTDTNLQCSTWPDSIPLLAGYSATPTFSFDPCTYGSYDPSAYFASNGIAGSMYTLPDQFP
RSENDMLDNSNTSNGNKSDKDGIKLEDEDEILEDEENDEEDDGTGKRKKRKRRVLFTKAQTYELERRFRS
QKYLSAPEREALAMQIRLTPTQVKIWFQNHRYKTKKSHTDKPINAALLTTMPNAFSSQSTAASFPTRAMP
IPMLVRDSSARSSDISSTSPYTVAFGSANSGYLPTPSAYLPATSGYFSNGPSAASSYMTNTQWWPS